THE ABILITY OF STAPHLOCOCCUS EPIDERMIDIS TO BUILD RESISTANCE TO TREATMENTS OF TRIFOLIUM REPENS
The purpose of this project was to determine if Staphlococcus epidermidis would build-up resistance to treatments of pure Trifolium repens (clover honey) over a short period of time. In order to produce results, three Petri dishes were inoculated with Staphlyococcus epidermidis, treated with a coffee filter soaked in pure honey, and put in an incubator over night. Due to the antiseptic properties of honey, the bacteria grew away from the coffee filter, leaving an empty area around the coffee filter. This area was measured and recorded before the bacteria closest to the coffee filter was placed on three more Petri dishes and placed in the incubator over night. After the empty areas on these plates were measured, the bacteria was placed on three new Petri dishes, placed in the incubator, and the empty areas measured. This entire experiment was run twice and the data combined, creating six values for trial one plates, six for trial two plates, and six trial three plates. An analysis of variation (ANOVA) statistical test was completed on the areas of the plates, comparing the areas of trial one to trial two and to trial three. The results of the ANOVA test concluded that the areas of the three plates were not equal. In fact, the areas decreased from trial one to trial three, which means that as time elapsed, the Staphylococcus epidermidis did build-up a resistance to treatments of Trifolium repens.
Research Done By:
Elizabeth Clement
Centerline High School
Cole Raxter
Centerline High School
Warren Consolidated Schools