The Effect of Sodium Hypochlorite, Ammonium Hydroxide, and Isopropyl Alcohol on the Growth of Eschericia Coli and Micrococcus Leteus

The objective of our project was to determine how effective bleach, ammonia, and isopropyl alcohol would be in eliminating colonies of Escherichia coli and Micrococcus luteus. To accomplish this, bacteria was grown on sterile agar plates, one of the three treatments was applied, and the number of bacteria colonies before and after treatment were recorded. We performed thirty trials for each treatment. Through research the optimum concentration for killing bacteria in the household was found. These optimum levels were 5% bleach, 4% ammonia, and 70% isopropyl alcohol. The results of our experiment did not come out as we had expected. The bacteria continued growing even after applying the treatments which we thought would kill most of the bacteria.

Research Done By:

Madeline Barick
Sterling Heights High School

James Geyman
Warren Woods Tower High School