The Effect of Technique, Solution and Grade of Filter Paper on the Efficiecnty and Accuracy of Paper Chromatography

Chromatography has many vital uses; therefore an accurate and cost efficient method must be determined. The experiment was carried out by performing the ascending strip technique, where the filter paper is placed vertically with the end in the solvent, and the horizontal circular technique, where the circular filter paper is placed horizontally on a Petri dish and the wick reaching down into the solvent, using solutions of 0.5M iron III nitrate and 0.5M copper nitrate and grades one, two, and three of Whatman filter papers. Five trials were completed for each combination in order to determine which combination was the most time efficient and accurate and which factor had the greatest effect on absorption time. The experiment concluded that all three factors had a significant effect on the absorption time; however, it was concluded that Grade 3 of Whatman filter paper was the most efficient regardless of the technique and solution used.

Research Done By:

Brittany Johns
L'Anse Creuse High School

Kristen Kokoszka
L'Anse Creuse High School- North